Journal: bioRxiv

Article Title: mScarlet3-H with low brightness and fluorescence lifetime has potential for cellular lifetime-unmixing and lifetime-based pH-sensing applications

doi: 10.1101/2025.07.15.664898

Figure Lengend Snippet: a. Relative brightness (average ± sd) of mScarlet3 and mScarlet3-H in HeLa cells 24 h after transfection, determined by normalized, spectrally corrected ratios of red to cyanfluorescence intensity found in cells upon co-production of RFP and mTurquoise2. b. maturation speed of mScarlet3-H in HeLa cells measured as delay (average ± sd in min) relative to co-produced mTurquoise2, n=8 cells. c. Photobleaching kinetics of mScarlet and mScarlet3 in living HeLa cells. d. Photobleaching kinetics of mScarlet-H and mScarlet3-H in living HeLa cells. For c. and d. cells were subjected to widefield microscopy and illuminated at ∼ 4W/cm2 with 550/15 nm LED power. Only backgroundfluorescence was subtracted and the netfluorescence intensity was normalized to the initial value at t=0. The average ± sd of four individual cells is shown for each curve.

Article Snippet: After 24 h, fluorescence images were collected using a Nikon Eclipse Ti-E widefield microscope with LEDs at 440 and 555 nm (SpectraX, Lumencor).

Techniques: Transfection, Produced, Microscopy

Journal: bioRxiv

Article Title: Cdc42 Partitioning by Chaperone Ydj1 During Asymmetric Division and Aging in Yeast

doi: 10.1101/2025.07.10.664052

Figure Lengend Snippet: Images were captured every 20 min at 27°C using an inverted widefield microscope (TiE, Nikon), and fluorescence images were deconvolved. The video shows frames for 56 hr 20 min of Cell 1, with selected time points shown in . The display rate is 8 frames per sec (fps).

Article Snippet: Microfluidics-assisted time-lapse imaging was performed using an inverted widefield fluorescence microscope (Ti-E; Nikon) equipped with a 60x/1.4 NA objective lens (5 z stacks, 0.5 μm step) and DIC optics (see above).

Techniques: Microscopy, Fluorescence